Skip to main content
فهرست مقالات

Regeneration and Agrobacterium-mediated transformation of three economically important strawberry cultivars Kurdistan, Camarosa and Paros مقاله


نویسنده مسئول:


Genetic transformation studies were carried out to standardize a protocol for Agrobacterium -mediated genetic transformation of three economically important strawberry (Fragaria x ananassa Duch) cultivars Kurdistan’, Camarosa’, and ‘Paros’. Shoot regeneration frequency 72, 65 and 30% was obtained on MS (1) basal medium supplemented with 2% glucose and 4 mg/l TDZ for Camarosa, Kurdistan and Paros, respectively. To optimize the concentration of kanamycin for these cultivars, we observed the responses of leaf explants to different concentration of kanamycin (0-100 mg/l) in the media. Our results showed that 75 mg/l was an effective concentration of kanamycin. For genetic transformation, Agrobacterium tumefaciens strain C58C1RifR (pGV2260) harbouring the binary vector pTJK136 containing uidA gene with intron along with kanamycin resistance gene (npt-II) was used. After five days pre-incubation and 72 h co-cultivation, transformed cells (explants) were able to grow on the selective regeneration medium containing 75 mg/l kanamycin and 500 mg/l cefotaxime, while, control explants failed to grow. The regenerated putative transgenic shoots were analyzed by histochemical GUS assay and PCR analysis. Transformation efficiency based on inoculated explants number was 2, 1 and 3% for ‘, Camarosa’, ‘Paros ’, and ‘Kurdistan’, respectively. The standardized protocol would be useful for Agrobacterium-mediated genetic transformation of these cultivars with important agronomic genes.

کلیدواژه ها:

GUS ، strawberry ، genetic transformation ، Agrobacterium tumifaciens

برای مشاهده محتوای مقاله لازم است ورود پایگاه شوید. در صورتی که عضو نیستید از قسمت عضویت اقدام فرمایید.

لمشاهدة محتوی المقال یلزم الدخول إلی دخول الموقع.
إن كنت لا تقدر علی شراء الاشتراك عبرPayPal أو بطاقة VISA، الرجاء ارسال رقم هاتفك المحمول إلی مدير الموقع عبر

You should become a Sign in to be able to see articles.
If you fail to purchase subscription via PayPal or VISA Card, please send your mobile number to the Website Administrator via